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  Yanli Wang, Ph.D, Prof.

  Principal Investigator

  Winner of Hundred talents plan, Chinese Academy of Science

  Winner of The National Science Fund for Distinguished Young Scholars

  Winner of Yangtse Rive Scholar Bonus Schemes

  Winner of Ten Thousand Talent Program

  Beijing Key Laboratory of Noncoding RNA, IBP

  Structure and function of the argonaute silencing complex

  E-mail:ylwang@ibp.ac.cn,Tel:010-64881316

  Fax:010-64881316,Zip code:100101

  Chinese personal homepage,  Group Website

Biography & Introduction

2002-2004 Ph.D, University of Science and Technology of China

2005-2006 Research Assistant, Institute of Biophysics, Chinese Academy of Sciences

2006-2011 Research Fellow, Research Associate, and Senior Research Scientist

                Memorial Sloan-Kettering Cancer Center, New York

2011-present Principal Investigator, Institute of Biophysics, Chinese Academy of Sciences

2015-present professor of University of Chinese Academy of Science

Wang’s Awards

HHMI international young scholar, 2017

Outstanding tutor of Chinese Academy of Science, 2017

The 13th Chinese young women scientist award, 2016 

Young and middle-aged outstanding talents, Ministry of Science and Technology, 2016 

The 9th Tanjiazhen Life Science awards(innovation) 

Chinese Annual Paper Awards, Cell Press, 2015 

Outstanding tutor of Chinese Academy of Science, 2015

Students’ Awards

Li xueyan and Yin maolu,  National master scholarship, 2017

Li jiazhi, Outstanding Award of director of Chinese Academy of Science, 2016

Li jiazhi, National master scholarship, 2016

Zhao hongtu, Outstanding Graduates Award, 2016

Chen peng, Scholarship of University of Chinese Academy of Science, 2016

Zhao hongtu, Special Award of Chinese Academy of Science, 2015

Zhao hongtu, National master scholarship, 2015

Zhao hongtu, Director Paper Award, 2015

The main 

Research Interest

The main research focus of our group is to understand how small regulatory RNA or DNAmediates prokaryotic defense against invasion by foreign nucleic acids. The main focus of my work is to systematically demonstrate the mechanisms of 1) the CRISPR-Cas system and 2) Argonaut (Ago) protein-mediated RNA/DNA interference.

►  CRISPR-Cas systems

The clustered regularly interspaced short palindromic repeats (CRISPR), together with CRISPR-associated (Cas) proteins form the microbial adaptive immune system that protects against invading phages and plasmids. The CRISPR-Cas system is a RNA-guided immune system found in nearly half of all bacteria studied, as well as in the majority of archaea. Our research in this area includes the molecular mechanism of spacer acquisition, pre-crRNA processing and RNA-guided DNA/RNA cleavage.The overall goal of our studies of the CRISPR-Cas system is to gain important insights into the structural-functional relationship of the processes prokaryotes use to fight invading nucleic acids.

►  Ago protein-mediated DNA interference

RNA interference is a conserved biological response to double-stranded RNA that regulates gene expression. The response is mediated by small interfering RNAs (siRNAs), which guide the sequence-specific degradation of cognate messenger RNAs (mRNAs).Ago protein is a key component in the RNA interference pathway. Recent bioinformatics analyses suggest that prokaryotic Ago plays a number of critical roles in the anti-viral defense system of bacteria. Unlike its eukaryotic counterpart where mRNA interference is mediated by siRNA, several pAgo proteins have been shown to perform either RNA-guided or DNA-guided interference of DNA.Our long-term goals are to structurally characterize and mechanistically define events associated with RNA or DNA interference.

Selected Publications

1. Yin M, Wang J, Wang M, Li X, Zhang M, Wu Q,  Wang, Y. Molecular mechanism of directional CTCF recognition of a diverse range of genomic sites. Cell Research, 2017, 27(11):1365-1377.

2. Liu L, Li X, Ma J, Li Z, You L, Wang J, Wang M, Zhang X, Wang, Y. The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a. Cell, 2017. 170(4):714-726.

3. Sheng G, Gogakos T, Wang J, Zhao, H, Serganov A, Juranek S, Tuschl T, Patel D,  Wang, Y. Structure/cleavage-based insights into helical perturbations at bulge sites within T. thermophilus Argonaute silencing complexes. Nucleic Acids Res. 2017, 45(15):9149-9163.

4. Liu L, Li X, Wang J, Yin M, Chen P, Wang M, Li J, Sheng G, Wang Y. Two Distant Catalytic Sites Are Responsible for C2c2 RNase Activities. Cell. 2017,168:121-134.

5. Liu L, Chen P, Wang M, Li X, Wang J, Yin M, Wang Y. C2c1-sgRNA Complex Structure Reveals RNA-guided DNA Cleavage Mechanism. Molecular Cell. 2017, 65.

6. Wang J, Ma J, Cheng Z, Meng X, You L, Wang M, Zhang X, Wang, Y. A CRISPR evolutionary arms race: structural insights into viral anti-CRISPR/Cas responses. Cell Research. 2016, 26:1165–1168.

7. Wang, J., Li, J., Zhao, H., Sheng, G., Wang, M., Yin, M. & Wang, Y., Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas System. Cell. 2015, 163: 840-853.

8. Zhao, H., Sheng, G., Wang, J., Wang, M., Bunkoczi, G., Gong, W., Wei, Z. & Wang, Y. “Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli”, Nature. 2014, 151: 147-150.

9. Swarts, D. C., Makarova, K., Wang, Y., Nakanishi, K., Ketting, R., Koonin, E., Patel, D. J. & Oost, van der, J., “The evolutionary journey of Argonaute proteins”, Nature structural & molecular biology, 2014, 21: 743-753.

10. Swarts, D. C., Jore, M. M., Westra, E. R., Zhu, Y., Janssen, J. H., Snijders, A. P., Wang, Y., Patel, D. J., Berenguer, J., Brouns, S. J.J. & Oost, van der, J. "DNA-guided DNA interference by a prokaryotic Argonaute", Nature. 2014, 507: 258-261.

11. Sheng, G., Zhao, H., Wang, J., Rao, Y., Tian, W., Swarts, D. C., van der Oost, J., Patel, D. J. and Wang, Y. "Structure-based cleavage mechanism of Thermus thermophilus Argonaute DNA guide strand-mediated DNA target cleavage." Proc Natl Acad Sci U S A.2014, 111(2): 652-657.

12. Swarts, D. C., Jore,M. M., Westra,E. R., Zhu, Y., Janssen, J. H., Snijders,A. P., Wang, Y., Patel, D. J., Berenguer, J., Brouns, S. J. and van der Oost, J. (2014). "DNA-guided DNA interference by a prokaryotic Argonaute." Nature 507(7491): 258-261.

13. Rüdel S, Wang, Y, Lenobel R, K?rner R, Hsiao HH, Urlaub H, Patel D, Meister G. Phosphorylation of human Argonaute proteins affects small RNA binding. Nucleic Acids Res. 2011,39:2330-43.

14. Wang, Y., Ludwig J., Schuberth C., Goldeck M., Schlee M., Li H., Juranek S., Sheng G., Micura R., Tuschl T., Hartmann G., Patel D., Structural and functional insights into 5'-ppp RNA pattern recognition by the innate immune receptor RIG-I. Nat Struct Mol Biol. 2010, 17:781-7.. 2010, 17:781-7.

15 .Wang, Y, Juranek S, Li H, Sheng G, Tuschl T, Patel DJ. Nucleation, propagation and cleavage of target RNAs in Ago silencing complexes. Nature. 2009, 461:754-761.

16. Wang, Y, Juranek S, Li H, Sheng G, Tuschl T, Patel DJ. Structure of an argonaute silencing complex with a seed-containing guide DNA and target RNA duplex. Nature. 2008, 56:921-926.

17. Wang, Y, Sheng G, Juranek S, Tuschl T, Patel DJ., Structure of the guide-strand-containing argonaute silencing complex. Nature. 2008, 456:209-213.

18. Wang, Y, Liu L, Wei Z, Cheng Z, Lin Y, Gong W. Seeing the process of histidine phosphorylation in human bisphosphoglycerate mutase. J Biol Chem. 2006, 281:39642-8.

19. Wang, Y, Wei Z, Liu L, Cheng Z, Lin Y, Ji F, Gong W. Crystal structure of human B type phosphoglycerate mutase bound with citrate. Biochem. Biophys. Res. Commun. 2005, 331:1207-15.

20. Wang, Y, Wei Z, Bian Q, Cheng Z, Wan M, Liu L, Gong W,Crystal structure of human bisphosphoglycerate mutase, J. Biol. Chem. 2004, 279: 39132-8.

 

 

From Yanli Wang, 2018-02-09 update

  

  

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Institute of Biophysics, CAS    Address: 15 Datun Road, Chaoyang District, Beijing, 100101, China
Tel:8610-64889872    Fax: 8610-64871293    E-mail: office@ibp.ac.cn