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Xiaohong Liu, Ph.D,  Prof.
Members of the Youth Innovation Promotion Association, CAS
Key Laboratory of  RNA Biology, IBP, CAS
Chemical biology and Bioinorganic chemistry  
Fax:010-64852570,Zip code:100101 
Chinese personal homepage

Biography & Introduction 

2002.9-2005.7 Ph.D. in Organic Chemistry, Chemistry Department, Tsinghua University
2005.8-2008.4 Postdoctoral researcher, Teikyo University of Science and Technology, Japan 
2008.5-at present, Institute of Biophysics, CAS 

The main interest study is to use small, soluble protein scaffold, and the genetic incorporation of unnatural amino acid to design easy-to-characterize, easy-to-produce, and easy-to-optimize metalloenzymes which catalyze these important reactions with equal or greater efficiency/selectivity than that of the natural systems. A series of studies of themetalloenzymes analog has been studied based on the genetic encodingtechnology. For the first time we have successfully designed a functional cytochrome c oxidase model that catalyzes selective and efficient oxygen reduction to water with the 18 kD myoglobin. Second, through the incorporation of metal-chelating amino acids into green fluorescent protein, photoinduced electron transfer was shown to occur within one nanosecond in a distance dependent manner. Such compounds can be used as power tools for studying of PET and using of biological components to achieve efficient and controllable light-induced charge separation.

Selected Publications 

1. Yu Y#, Liu X#, Wang J*. Expansion of Redox Chemistry in Designer Metalloenzymes. AccChem Res. doi: 10.1021/acs.accounts.8b00627. [Epub ahead of print]

2. Liu X#, Kang F#, Hu C#, Wang L, Xu Z, Zheng D, Gong W, Lu Y, Ma Y, Wang J*. A genetically encoded photosensitizer protein facilitates the rational design of a miniature photocatalytic CO2-reducing enzyme. Nat Chem. 2018, 10 (12):1201-1206. doi: 10.1038/s41557-018-0150-4.

3. Mu Z, Zou Z, Yang Y, Wang W, Xu Y, Huang J, Cai R, Liu Y, Mo Y, Wang B, Dang Y, Li Y, Liu Y, Jiang Y, Tan Q, Liu X, Hu C, Li H, Wei S, Lou C, Yu Y, Wang J*. A genetically engineered Escherichia coli that senses and degrades tetracycline antibiotic residue. Synth SystBiotechnol. 2018, 3(3):196-203. doi: 10.1016/j.synbio.2018.05.001.

4. Wang L#, Chen X#, Guo X#, Li J, Liu Q, Kang F, Wang X, Hu C, Liu H, Gong W, Zhuang W, Liu X*, Wang J*. Significant expansion and red-shifting of fluorescent protein chromophore determined through computational design and genetic code expansion. Biophys Rep. 2018, 4(5):273-285. doi: 10.1007/s41048-018-0073-z.

5. Yu Y#, Cui C#, Liu X#, Petrik ID, Wang J*, Lu Y*. A Designed Metalloenzyme Achieving the Catalytic Rate of a Native Enzyme. J Am Chem Soc. 2015, 137(36):11570-3. doi: 10.1021/jacs.5b07119.

6. Yu Y, Hu C, Liu X, Wang J*. Synthetic Model of the Oxygen-Evolving Center: Photosystem II under the Spotlight. Chembiochem. 2015, 16(14):1981-3. doi: 10.1002/cbic.201500302.

7. Lv X#, Yu Y#, Zhou M, Hu C, Gao F, Li J, Liu X, Deng K, Zheng P, Gong W, Xia A*, Wang J*. Ultrafast photoinduced electron transfer in green fluorescent protein bearing a genetically encoded electron acceptor. J Am Chem Soc. 2015, 137(23):7270-3. doi: 10.1021/jacs.5b03652.

8. Yang Y#, Zhou Q#, Wang L#, Liu X, Zhang W, Hu M, Dong J, Li J, Xiaoxuan L, Ouyang H, Li H, Gao F, Gong W, Lu Y*, Wang J*. Significant Improvement of Oxidase Activity through the Genetic Incorporation of a Redox-active Unnatural Amino Acid. Chem Sci. 2015, 6(7):3881-3885. doi: 10.1039/C5SC01126D

9. X.H. Liu, L Jiang1, J Li, L Wang, Y Yu, Q Zhou, X Lv, W Gong, Y Lu, JY Wang. Significant Expansion of Fluorescent Protein Sensing Ability through the Genetic Incorporation of Superior Photo-Induced Electron-Transfer Quenchers. J. Am. Chem. Soc. 2014 Sep, 136, 13094-13097. 

10. X.H. Liu, J.S. Li C. Hu, Q. Zhou, W. Zhang, M.R. Hu, J.Z. Zhou, J.Y. Wang. Significant Expansion of the Fluorescent Protein Chromophore through the Genetic Incorporation of a Metal-chelating Unnatural Amino Acid. Angew Chem Int Ed Engl. (2013), 52, 4805-9(VIP and Back Cover Article)

11. X.H. Liu, J.S. Li1, J.S. Dong, C. Hu, W.M. Gong, and J.Y. Wang.Genetic Incorporation of a Metal Chelating Amino Acid as a Probe for Protein Electron Transfer. Angew Chem Int Ed Engl. (2012)Oct 8;51(41):10261-5(Hot Paper, Back Cover Article, recommended by F1000 biology)

12. X.H. Liu, Y. Yu1, C. Hu, W. Zhang, Y. Lu, and J.Y. Wang. Significant Increase of Oxidase Activity through the Genetic Incorporation of a Tyrosine-Histidine Cross-Link in a Myoglobin Model of Heme-Copper Oxidase. Angew Chem Intl Ed(2012),51 (18), 4312-4316 (Frontispiece, News of the Week, Chemical and Engineering News)

13. X.H.Liu, T. Yamaguchi, M, Saneyoshi et al. Telomerase inhibition by 3'-Azido-2',3'-dideoxynucleoside 5'-triphosphates inhibit telomerase activity in vitro, and the corresponding nucleosides cause telomere shortening in human HL60 cells. Nucleic Acid Research, 35(21), (2007), 7140-7149


From Xiaohong Liu, 2019-03-25 update


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Institute of Biophysics, CAS    Address: 15 Datun Road, Chaoyang District, Beijing, 100101, China
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