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  Kelong Fan, Ph. D, Professor
 
  PI (IBP-Fellow)
      Member of the Youth Innovation Promotion Association of CAS
  Young Elite Scientist Sponsorship Program by CAST
  Institute of Biophysics, Chinese Academy of Sciences
  Nanobiology
  E-mail: fankelong(AT)ibp.ac.cn 
  Tel: +86-10-64888280
  Fax: +86-10-64888584
  Zip code: 100101

Biography & Introduction

Education & Positions

2005.09-2009.07  B.Sc. in Bioengineering, China University of Mining and Technology (Beijing), Beijing;

2009.09-2014.07  Ph.D. in Cell Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing;

2014.08-2017.12  ‘Morningside-IBP’ Postdoctoral Program, Institute of Biophysics, Chinese Academy of Sciences, Beijing;

2018.01-2019.07 Associate Professor, Institute of Biophysics, Chinese Academy of Sciences, Beijing.

2019.07-present  Professor, Institute of Biophysics, Chinese Academy of Sciences, Beijing.

Research Interest  & Recent progresst

My research interests focus on the novel functions and applications of nanozyme and ferritin in biomedicine. We discovered new functions of H chain ferritin (HFn) and made a big breakthrough in applications of nanozyme combining new features of HFn. Since then, much original and significant progress has been achieved.
Human HFn targeting tumor and traversing BBB: We firstly discovered that human HFn can target and visualize tumor via binding specifically to a tumor targeting marker transferrin receptor 1 (TfR1) (Nature Nanotechnology 2012;PNAS, 2014). Further studies showed that ferritin traverses blood brain barrier and accumulates in the lysosomal compartment of tumor cells after binding to TfR1 (ACS Nano, 2018). Those findings build up a basis for ferritin nanocarriers and nanozymes in new technologies and methods for tumor diagnosis and therapy.
Ferritin as nanocarrier: Combined with the new characteristics of ferritin, Ferritin Drug Carriers were developed, providing a potential nano-drug platform for central nervous system diseases and malignant tumors.
Another method of loading drugs into ferritin relies on its self-assembly ability. Nanobodies will be displayed on the surface of ferritin after its self-assembly, resulting in high affinity and half-life extension (Analytical Chemistry, 2018).
Novel detection methods based on ferritin and nanozyme: Taking advantages of nanozyme activity and magnetism property of iron oxide nanozyme, we developed a novel rapid, highly sensitive, and easy-to-use nanozyme-strip for emergency infectious disease detection. (Biosensor Bioelectronics, 2015).
In addition, we developed a new type of probes for cancer diagnosis based on nanozyme and ferritin. Working with clinicians, we examined 1400 specimens from patients with ten types of cancer and verified that these probes boast high sensitivity and specificity, which are qualified for clinical use (Nature Nanotechnology, 2012).
Improvements, designs and applications of nanozyme: Compared with natural enzymes, nanozyme boasts many advantages but its activity and selectivity need to be improved. To optimize its catalytic activity, we introduced Histidine residues onto the Fe3O4 nanoparticle surface in order to mimic the enzymatic microenvironment of natural peroxidase enzymes. The catalytic efficiency was enormously enhanced (Chemical Communications,2017).
In addition, we designed a nanozyme using nitrogen-doped porous carbon nanospheres which possess four enzyme-like activities (oxidase, peroxidase, catalase and superoxide dismutase). We then introduce ferritin to this multifunctional nanozyme into lysosomes and boost reactive oxygen species (ROS) generation in a tumor-specific manner. In the acidic environment of tumor lysosomes, oxidase and peroxidase activity were specifically activated and the dissolved oxygen and hydrogen peroxide generated in tumor cells were directly converted into ROS, which can directly kill tumor cells. This work provides a new concept of nanozyme-mediated tumor catalytic therapy (Nature Communications, 2018).

Selected Publications

1. Kelong Fan, Changqian Cao, Yongxin Pan, Di Lu, Dongling Yang, Jing Feng, Lina Song, Minmin Liang and Xiyun Yan. Magnetoferritin nanoparticles for targeting and visualizing tumour tissues.Nature Nanotechnol. 2012, 7, 459-464.
(Highlighted by Gen. Eng. Biotechnol. News: Magnetoferritin Nanoparticles Highlight Tumors Without Targeting Ligands or Contrast Agents)
(Highlighted by Chemistry Views: Detecting Tumor Cells with Iron)
(Included by Wikipedia ‘Artificial enzyme’)
2. Kelong Fan#, Juqun Xi#, Lei Fan, Peixia Wang, Chunhua Zhu, Yan Tang, Xiangdong Xu, Minmin Liang, Bing Jiang, Xiyun Yan#, Lizeng Gao#. In vivo Guiding Nitrogen-doped Carbon Nanozyme for Tumor Catalytic Therapy. Nature Commun.2018, 9: 1440.
(Highlighted by Global Times, The New Indian Express, Business Standard, andChina Daily: Chinese scientists develop nanozymes to target tumor cells; Nanozyme strategy helps kill tumors)
(Included by Wikipedia ‘Artificial enzyme’)
3. Kelong Fan*#, Xiaohua Jia#, Meng Zhou#, Kun Wang, Jo?o Conde, Jiuyang He, Jie Tian* and Xiyun Yan*. Ferritin Nanocarrier Traverses the Blood Brain Barrier and Kills Glioma. ACS Nano 2018, 12(5): 4015-4115. (*Corresponding authors).
(Selected as Breakthrough of nanotechnology in 2018 by New Trends in Nanotechnology, Material and Environmental sciences)
4. Minmin Liang#, Kelong Fan#, Meng Zhou, Demin Duan, Jiyan Zheng, Dongling Yang, Jing Feng and Xiyun Yan. H-ferritin-nanocaged doxorubicin nanoparticles specifically target and kill tumors with a single-dose injection. Proc. Natl. Acad. Sci. USA 2014, 111(41): 14900-14905 (#Contributed equally to this work).
(Highlighted by Asia Scientists: Harnessing Nature's Design To Reduce Chemo Side-Effects)
5. Hui Ding, Yanjuan Cai, Lizeng Gao, Minmin Liang, Beiping Miao, Hanwei Wu, Yang Liu, Ni Xie, Aifa Tang, Kelong Fan*, Xiyun Yan*, and GuohuiNie*. Exosome-like Nanozyme Vesicles for H2O2-responsive Catalytic Photoacoustic Imaging of Xenograft Nasopharyngeal Carcinoma. Nano Letters. 2019,19(1): 203-209. (*Corresponding authors)
6. Bolong Xu#, Hui Wang#, Weiwei Wang#, Lizeng Gao, Shanshan Li, Xueting Pan, Hongyu Wang, Hailong Yang, Xiangqin Meng, Qiuwen Wu, Lirong Zheng, Shenming Chen, Xinghua Shi, Kelong Fan*, Xiyun Yan*, and Huiyu Liu*. Single-Atom Nanozyme for Wound Antibacterial Applications. Angew. Chem. Int. Ed. 2019,
https://doi.org/10.1002/anie.201813994.(*Corresponding
authors)
(Highlighted by Chemical & Engineering
News: MOF-derived
nanoparticles exhibit enzymelike catalytic activity)
7. Bing Jiang, Ruofei Zhang, Jianlin Zhang, Yaxin Hou, Xuehui Chen, Meng Zhou, Xiuyun Tian, Chunyi Hao, Kelong Fan*, and Xiyun Yan*. GRP78-targeted ferritin nanocaged ultra-high dose of doxorubicin for hepatocellular carcinoma therapy. Theranostics. doi:10.7150/thno.30867. (*Corresponding authors)
8. Demin Duan#, Kelong Fan#, Dexi Zhang, Shuguang Tan, Mifang Liang, Yang Liu, Jianlin Zhang, Panhe Zhang, Wei Liu, XiangguoQiu, Gary P. Kobinger, George Fu Gao and Xiyun Yan. Nanozyme-strip for rapid local diagnosis of Ebola. Biosens. Bioelectron. 2015, 74: 134-141 (#Contributed equally to this work).
(Highlighted by ELSEVIER: How a new test could help curb the spread of Ebola)
(Selected to be awarded the Elsevier Atlas)
(Included by Wikipedia ‘Artificial enzyme’)
9. Bing Jiang#, Liang Yan#, Jianlin Zhang, Meng Zhou, Guizhi Shi, Xiuyun Tian, Kelong Fan*, Chunyi Hao*, Xiyun Yan*. Biomineralization Synthesis of the Cobalt Nanozyme in SP94-Ferritin Nanocages for Prognostic Diagnosis of Hepatocellular Carcinoma. ACS Appl. Mater. Interfaces 2019, 11(10), 9747-9755. (*Corresponding authors)
10. Jiuyang He, Kelong Fan* and Xiyun Yan*. Ferritin Drug Carrier (FDC) for tumor targeting therapy. Journal of Controlled Release. 2019. Doi.org/10.1016/j.jconrel.2019.09.002.(*Corresponding authors)
11. Yiliang Jin, Jiuyang He, Kelong Fan*, and Xinyun Yan*. Ferritin variants: Inspirations for rationally designing protein nanocarriers. Nanoscale. 2019, 11, 12449-12459. (*Corresponding authors)
12. Kelong Fan, Hui Wang, Juqun Xi, Qi Liu, Xiangqin Meng, Demin Duan, Lizeng Gao, Xiyun Yan. Optimization of Fe3O4 nanozyme activity via single amino acid modification mimicking an enzyme active site. Chem. Commun.(Camb.) 2017, 53(2): 424-427.
(Included by Wikipedia ‘Artificial enzyme’)
13. Kelong Fan#*, Bing Jiang#, Zhe Guan, Jiuyang He, Dongling Yang, Ni Xie, GuohuiNie, Can Xie, and Xiyun Yan*. Fenobody: A Ferritin-Displayed Nanobody with High Apparent Affinity and Half-Life Extension. Anal. Chem.2018, 90 (9): 5671–5677.(*Corresponding authors)
14. Yang Du#, Kelong Fan#, Hejun Zhang, Li Li, Peixia Wang, Jiuyang He, Shigang Ding, Xiyun Yan, Jie Tian. Endoscopic molecular imaging of early gastric cancer using fluorescently labeled human H-ferritin nanoparticle. Nanomedicine: NBM. 2018, 14 (7): 2259-2270 (#Contributed equally to this work)
15. Kelong Fan, Lizeng Gao and Xiyun Yan. Human ferritin for tumor detection and therapy. WIREs Nanomed. Nanobiotech. 2013, 5, 287-298.
16. Kelong Fan, Meng Zhou, Xiyun Yan. Questions about horse spleen ferritin crossing the blood brain barrier via mouse transferrin receptor 1. Protein & Cell. 2017, 8 (11): 788-790.
17. Xiangqin Meng, Kelong Fan*. Application of Nanozymes in Disease Diagnosis. Progress in Biochemistry and Biophysics. 2018. 45(2): 218-236. (# Corresponding authors)
18. Kelong Fan, Meng Zhou, Xiyun Yan. Novel properties and applications of human ferritin expressed by Escherichia coli. Microbiol. China 2014, 41, 520-538.
Cahpters

1. Kelong Fan, Minmin Liang, Xiyun Yan, Chapter 3: The novel properties of ferritin and its application in nanomedicine. “Nanomaterials: emerging characteristics and biomedicalapplications” (Ed.: Xiyun Yan), 2014, Science Press (in Chinese).
Selected for inclusion in the Collection of Nano Science and Technology.
2. Kelong Fan, Xiyun Yan. Chapter 6: Bioengineered ferritin nanoprobes for cancer theranostics. ‘Handbook of Nanomaterials for Cancer Theranostics’(Ed.: Joao Conde) 2018.ELSEVIER.
3. Kelong Fan. Chapter 9: Cerium oxide based nanozymes; Chapter 13 Nanozyme based tumor theranostics. ‘Nanozymes: Connecting Biology and Nanotechnology’.(Ed.: Xiyun Yan) Springer. 2019 (Invited).
4. Kelong Fan. Chapter: Nanozymes and their applications in biomedicine. ‘21ST CENTURY NANOSCIENCE: A Handbook’ (Ed.: Klaus D. Sattler) TAYLOR & FRANCIS. 2019 (Invited).
5. Kelong Fan. Chapter: Nanozymes in the treatment of neurological diseases. ‘The Application of Nanomedicine in Brain Disease’ (Ed.: XueXue)

Academic Participation
1. Invited Reviewer for Nano Today, ACS Nano、Nano Letters、Biomaterials、Small、Biosensor Bioelectronics、Analytical Chemistry、Bioconjugate Chemistry、Materials Science & Engineering C、Scientific Reports.
Excellent Reviewer for Materials Science & Engineering Cin 2018。
2. Invited Guest Editor forJournalof Oncology。
3. Member of the Youth Committee of Nano-Tumor Professional Committee, Chinese Anti-Cancer Sssociation。
4. Member of the Youth Committee of Nano-Biology branch of Chinese Society of Biophysics。

 

  

From Kelong Fan, 2019-09-30

    

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