Kelong Fan, Ph.D, Prof.
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Principal Investigator (IBP-Fellow)
State Key Laboratory of Biomacromolecules, IBP
Young Elite Scientist Sponsorship Program by CAST
Research Interests: Nanobiology
Email: fankelong@ibp.ac.cn
Tel: 010-64888280
Address: 15 Datun Road, Chaoyang District, Beijing, 100101, China
Chinese personal homepage
- Biography
2005.09 - 2009.07 B.Sc. in Bioengineering, China University of Mining and Technology (Beijing), Beijing
2009.09 - 2014.07 Ph.D. in Cell Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing
2014.08 - 2017.12 ‘Morningside-IBP’ Postdoctoral Program, Institute of Biophysics, Chinese Academy of Sciences, Beijing
2018.01 - 2019.07 Associate Professor, Institute of Biophysics, Chinese Academy of Sciences, Beijing
2019.07 - Professor, Institute of Biophysics, Chinese Academy of Sciences, Beijing
- Awards
- Membership in Academies & Societies
1. Invited Reviewer for Materials Today, Advanced Materials, Angew. Chem. Int. Ed., J. Am. Chem. Soc., Nano Today, ACS Nano, Nano Letters, Biomaterials, Small, Biosensor Bioelectronics, Analytical Chemistry, Bioconjugate Chemistry and Materials Science & Engineering C.
Excellent Reviewer for Materials Science & Engineering C in 2018.
2. Invited Guest Editor for Frontiers in Chemistry, Journal of Oncology.
3. Member and deputy secretary general of Nanozyme Branch of Chinese Biophysical Society.
4. Member of Material Biology and Intelligent Diagnosis and Treatment Sub-committee of Chinese Biophysical Society.
5. Member of Angiography Sub-committee of China Medical biotechnology Association.
6. Member of the Youth Committee of Nano-Tumor Professional Committee, Chinese Anti-Cancer Association.
7. Member of the Youth Committee of Nano-Biology branch of Chinese Society of Biophysics.
- Research Interests
My research interests focus on the novel functions and applications of nanozyme and ferritin in biomedicine. We discovered new functions of H chain ferritin (HFn) and made a big breakthrough in applications of nanozyme combining new features of HFn. Since then, much original and significant progress has been achieved.
Human HFn targeting tumor and traversing BBB: We firstly discovered that human HFn can target and visualize tumor via binding specifically to a tumor targeting marker transferrin receptor 1 (TfR1) (Nature Nanotechnology 2012；PNAS, 2014). Further studies showed that ferritin traverses blood brain barrier and accumulates in the lysosomal compartment of tumor cells after binding to TfR1 (ACS Nano, 2018). Those findings build up a basis for ferritin nanocarriers and nanozymes in new technologies and methods for tumor diagnosis and therapy.
Ferritin as nanocarrier: Combined with the new characteristics of ferritin, Ferritin Drug Carriers were developed, providing a potential nano-drug platform for central nervous system diseases and malignant tumors.
Another method of loading drugs into ferritin relies on its self-assembly ability. Nanobodies will be displayed on the surface of ferritin after its self-assembly, resulting in high affinity and half-life extension (Analytical Chemistry, 2018).
Novel detection methods based on ferritin and nanozyme: Taking advantages of nanozyme activity and magnetism property of iron oxide nanozyme, we developed a novel rapid, highly sensitive, and easy-to-use nanozyme-strip for emergency infectious disease detection. (Biosensor Bioelectronics, 2015).
In addition, we developed a new type of probes for cancer diagnosis based on nanozyme and ferritin. Working with clinicians, we examined 1400 specimens from patients with ten types of cancer and verified that these probes boast high sensitivity and specificity, which are qualified for clinical use (Nature Nanotechnology, 2012).
Improvements, designs and applications of nanozyme: Compared with natural enzymes, nanozyme boasts many advantages but its activity and selectivity need to be improved. To optimize its catalytic activity, we introduced Histidine residues onto the Fe3O4 nanoparticle surface in order to mimic the enzymatic microenvironment of natural peroxidase enzymes. The catalytic efficiency was enormously enhanced (Chemical Communications，2017).
In addition, we designed a nanozyme using nitrogen-doped porous carbon nanospheres which possess four enzyme-like activities (oxidase, peroxidase, catalase and superoxide dismutase). We then introduce ferritin to this multifunctional nanozyme into lysosomes and boost reactive oxygen species (ROS) generation in a tumor-specific manner. In the acidic environment of tumor lysosomes, oxidase and peroxidase activity were specifically activated and the dissolved oxygen and hydrogen peroxide generated in tumor cells were directly converted into ROS, which can directly kill tumor cells. This work provides a new concept of nanozyme-mediated tumor catalytic therapy (Nature Communications, 2018).
- Grants
- Selected Publications
1. Long Ma, Jia-Jia Zheng, Ning Zhou, Ruofei Zhang, Long Fang, Yili Yang, Xingfa Gao, Chunying Chen, Xiyun Yan*, Kelong Fan*. A natural biogenic nanozyme for scavenging superoxide radicals. Nature Communications. 2024,15, 233.
2. Xiangqin Meng, Huizhen Fan, Lei Chen, Jiuyang He, Chaoyi Hong, Jiaying Xie, Yinyin Hou, Kaidi Wang, Xingfa Gao, Lizeng Gao, Xiyun Yan*, Kelong Fan*. Ultrasmall metal alloy nanozymes mimicking neutrophil enzymatic cascades for tumor catalytic therapy. Nature Communications. 2024, 15, 1626.
3. Wei Jiang, Qing Li*, Ruofei Zhang, Jianru Li, Qianyu Lin, Jingyun Li, Xinyao Zhou, Xiyun Yan*, Kelong Fan*. Chiral metal-organic frameworks incorporating nanozymes as neuroinflammation inhibitors for managing Parkinson's disease. Nature Communications, 2023, 14, 8137.
4. Wenhui Gao, Jiuyang He, Lei Chen, Xiangqin Meng, Yana Ma, Liangliang Cheng, Kangsheng Tu, Xingfa Gao, Cui Liu*, Mingzhen Zhang*, Kelong Fan*, Dai-Wen Pang*, Xiyun Yan*. Deciphering the catalytic mechanism of superoxide dismutase activity of carbon dot nanozyme. Nature Communications. 2023, 14, 160.（ESI高被引论文）
5. Ruofei Zhang, Bing Jiang, Kelong Fan*, Lizeng Gao*, Xiyun Yan*. Designing nanozymes for in vivo applications. Nature Reviews Bioengineering. 2024, 2(10): 849-868.
6. Shuyu Wang, Miaomiao Cheng, Shenghui Wang, Wei Jiang, Feifei Yang, Xiaomei Shen, Lirong Zhang, Xiyun Yan*, Bing Jiang*, Kelong Fan*, A Self-Catalytic NO/O2 Gas-Releasing Nanozyme for Radiotherapy Sensitization through Vascular Normalization and Hypoxia Relief. Advanced Materials. 2024, 36(39): 2403921.
7. Zhuoran Wang, Yue Zhao, Yaxin Hou, Guoheng Tang, Ruofei Zhang, Yili Yang, Xiyun Yan*, Kelong Fan*. A Thrombin-Activated Peptide-Templated Nanozyme for Remedying Ischemic Stroke via Thrombolytic and Neuroprotective Actions. Advanced Materials. 2024, 36 (10): 202210144.（ESI高被引论文）
8. Daji Wang*, Jie Wang*, Xuejiao J. Gao, Hui Ding, Ming Yang, Zhiheng He, Jiaying Xie, Zixia Zhang, Haibing Huang, Guohui Nie*, Xiyun Yan*, Kelong Fan*. Employing Noble Metal-Porphyrins to Engineer Robust and Highly Active Single-Atom Nanozymes for Targeted Catalytic Therapy in Nasopharyngeal Carcinoma. Advanced Materials. 2024, 36(7), 2310033.
9. Jingqi Li, Xinda Cai, Peng Jiang, Huayuan Wang, Shiwei Zhang, Tiedong Sun*, Chunxia Chen*, Kelong Fan*. Co-based Nanozymatic Profiling: Advances Spanning Chemistry, Biomedical, and Environmental Sciences. Advanced Materials, 2024, 36(8), 2307337.
10. Yongjuan Li, Yu Zhang, Ya Dong, Ozioma Udochukwu Akakuru, Xiaohan Yao, Jinmeng Yi, Xinyan Li, Linlin Wang, Xiaohan Lou, Baoyu Zhu, Kelong Fan*, Zhihai Qin*. Ablation of Gap Junction Protein Improves the Efficiency of Nanozyme-Mediated Catalytic/Starvation/Mild-Temperature Photothermal Therapy. Advanced Materials, 2023, 22, 2210464.
11. Ruofei Zhang#, Bai Xue#, Yanhong Tao#, Hanqing Zhao, Zixia Zhang, Xiaonan Wang, Xinyao Zhou, Bing Jiang, Zhenglin Yang*, Xiyun Yan*, and Kelong Fan*. Edge-site Engineering of Defective Fe-N4 nanozymes with Boosted Catalase-like Performance for Retinal Vasculopathies. Advanced Materials. 2022, 34(39): 2205324.（ESI高被引论文）
(From Kelong Fan, November 21, 2024)