2001.09 - 2005.06  B.Sc. in Biomedical engineering, Huazhong University of Science and Technology, China

2005.09 - 2010.07  Ph.D. in Biophsics, Institute of Biophysics, CAS

2010.08 - 2011.12  Assistant professor, Institute of Biophysics, CAS

2012.01 - 2016.12  Associate professor, Institute of Biophysics, CAS

2017.01 - 2020.07  Professor, Institute of Biophysics, CAS

2020.07 -                Professor, Principal Investigator, Institute of Biophysics, CAS

Try to develop new single molecule localization microscopy and single molecule detection (SMD) methods to solve problems that now faced by biomedical researchers.

1. Luo S^#, Zhao X^#, Li Y^#, Fan C^#, Liu R, Gong R, Li W, Ma N, Yang Z, Xu T*, Ji W*, Gu L*. Molecular-scale isotropic 3D super-resolution microscopy via interference localization. Nat. Methods. 23, 183-192 (2026).

2. Yang T^#, He J^#, Zhao X^#, Ren C^#, Ding Z, Wang L, Zhao H, Chu L, Luo S, Shi C, Gu L, Xu T*, Yang G*, Ji W*. Self-adaptive fine-tuning of deep learning super-resolution microscopy for artifact suppression in live-cell imaging. The Innovation. 7(2), 101123 (2026),

3. Ren C^#, Pan T^#, Yang T^#, Yin N, Gu L, Liu B*, Ji W*. Combined learning for augmented super-resolution imaging. Opt. Laser Technol. 192, 113943 (2025).

4. Xiong S^#, Peng L^#, Gu L*, Zou P*, Ji W*. High pixel throughput voltage imaging based on repetitive optical selective exposure (ROSE). Optica.12(6), 860-871 (2025).

5. Ding Z^#, Zhao X^#, Wang L^#, Luo S, Yang T, Fan C, Lu J, Xiong S, Li W, Xu T*, Gu L*, Ji W*. Three-dimensional super-resolution imaging of whole cells using a high numerical aperture oblique-plane microscope. Opt. Lett. 50(11), 3529-3532 (2025).

6. Yang J^#, Tian B^#, Wang P^#, Chen R^#, Xiao K, Long X, Zheng X, Zhu Y, Sun F, Shi Y, Jiu Y, Ji W*, Xue Y*, Xu T*, Li Z*. SARS-CoV-2 NSP3/4 control formation of replication organelle and recruitment of RNA polymerase NSP12. J Cell Biol. 224 (3), e202306101 (2025).

7. Luo S^#, Gong R^#, Zhang M, Xu T*, Ji W*, Gu L*. Fast axial resolution enhancement microscopy with structured illumination. APL Photonics. 9, 126116 (2024).

8. Li W^#, Lu J^#, Xiao K^#, Zhou M^#, Li Y, Zhang X, Li Z, Gu L, Xu X, Guo Q*, Xu T*, Ji W*. Integrated multimodality microscope for accurate and efficient target-guided cryo-lamellae preparation. Nat. Methods. 20, 268-275 (2023).

9. Wu W^#, Luo S^#, Fan C^#, Yang T, Zhang S, Meng W, Xu T*, Ji W*, Gu L*. Tetra-color superresolution microscopy based on excitation spectral demixing. Light-Sci. Appl. 12, 9 (2023).

10. Zhang R^#, Gu L^#, Chen W^#, Tanaka N, Zhou Z, Xu H, Xu T, Ji W*, Liang X*, Meng W*. CAMSAP2/3 localize at microtubule intersections to regulate the spatial distribution of microtubules. J. Mol. Cell Biol. Accepted (2023).

11. Liu J^#, Tang H^#, Xu C, Zhou S, Zhu X, Li Y, Prézeau L, Xu T, Pin J*, Rondard P*, Ji W*, Liu J*. Biased signaling due to oligomerization of the G protein-coupled platelet-activating factor receptor. Nat. Commun. 13, 6365 (2022).

12. Wang L^#, Lu J^#, Ji W*, Wan L*, Gu L*. Interferometrical single-molecule localization based on dynamic PSF engineering. Opt. Lett. 47, 1770-1773 (2022).

13. Gu L, Ji W*. Recent progress on single-molecule localization microscopy. Biophys. Rep. 7, 365-376(2021).

14. Gu L^#, Li Y^#, Zhang S^#, Zhou M, Xue Y, Li W, Xu T*, Ji W*. Molecular-scale axial localization by repetitive optical selective exposure. Nat. Methods. 18, 369-373(2021).

15. Gu L^#, Li Y^#, Zhang S^#, Xue Y, Li W, Li D, Xu T*, Ji W*. Molecular resolution imaging by repetitive optical selective exposure. Nat. Methods. 16, 1114-1118(2019).

16. Li Y, Xue Y, Xu X, Wang G, Liu Y, Wu H, Li W, Wang Y, Chen Z, Zhang W, Zhu Y, Ji W, Xu T, Liu L*, Chen Q*. A mitochondrial FUNDC1/HSC70 interaction organizes the proteostatic stress response at the risk of cell morbidity. EMBO J. 38(3), e98786(2018).

17. Xu X, Xue Y, Tian B, Feng F, Gu L, Li W, Ji W*, Xu T*. Ultra-stable super-resolution fluorescence cryo-microscopy for correlative light and electron cryo-microscopy. Sci. China Life Sci. 61(11), 1312-1319(2018).

18. Zou W, Fu J, Zhang H, Du K, Huang W, Yu J, Li S, Fan Y, Baylis HA, Gao S, Xiao R, Ji W*, Kang L*, Xu T*. Decoding the intensity of sensory input by two glutamate receptors in one C. elegans interneuron. Nat. Commun. 9(1), 4311(2018).

19. Lan X^#, Fan C^#, Ji W, Tian F, Xu T*, Gao Z*. Grafting voltage and pharmacological sensitivity in potassium channels. Cell Res. 26, 935-945 (2016).

20. Liu B^#, Xue Y^#, Zhao W^#, Chen Y, Fan C, Gu L, Zhang Y, Zhang X, Sun L, Huang X, Ding W, Sun F, Ji W*, Xu T*. Three-dimensional super-resolution protein localization correlated with vitrified cellular context. Sci. Rep. 5, 13017(2015).

21. Gu L^#, Sheng Y^#, Chen Y^#, Chang H, Zhang Y, Lv P, Ji W*, Xu T*. High-density 3D single molecular analysis based on compressed sensing. Biophys J. 106(11), 2443-9(2014).

22. Zhang Y^#, Gu L^#, Chang H^#, Ji W^#, Chen Y, Zhang M, Yang L, Liu B, Chen L*, Xu T*. Ultrafast, accurate, and robust localization of anisotropic dipoles. Protein & Cell. 4(8), 598-606(2013).

(From Wei Ji, March 6, 2026)