Chinese Academy of
Sciences Protein
Science core facility
Center |
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IBP Seminar: THE RIBOSOME AS A BROWNIAN MACHINE |
| Author: |
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Update time: |
2012-09-04 |
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| what: |
THE RIBOSOME AS A BROWNIAN MACHINE |
| who: |
Joachim Frank, Professor, Member of National Academy of Sciences
Howard Hughes Medical Institute, Columbia University |
| when: |
2012-09-07 10:00am |
| where: |
Lecture Hall in Library, Institute of Biophysics |
| More Information |
Cryo-EM and single-molecule FRET of a sample of the pre-translocational ribosome (i.e. after peptidyl transfer), in the absence of EF-G, strongly suggest that the ribosome is a Brownian ratchet machine driven by thermal motion. In this picture the role of EF-G is relegated to that of a “pawl.” These insights have become possible as cryo-EM has attained its full power with the addition of classification tools that are able to pull out sub-populations of molecules in distinct intermediate states out of images depicting a pool of freely equilibrating molecules. We refer to this new capability by the term "Story in a Sample."
In addition, the most recent results obtained in our lab will be highlighted: a 5-A reconstruction of the ribosome from T. brucei, the parasite causing sleeping sickness, leading to a complete atomic model. |
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