Science Advances, 10 June, 2020, DOI：https://doi.org/10.1126/sciadv.aba0777

R-loops coordinate with SOX2 in regulating reprogramming to pluripotency

Yaoyi Li#, Yawei Song#, Wei Xu,#, Qin Li#, Xinxiu Wang Kuan Li, Juehan Wang Zicong Liu Sergiy Velychko, Rong Ye, Qing Xia Lei Wang, Rong Guo Xiaotao Dong Zhikai Zheng Yushuang Dai Haojie Li Mingze Yao, Yuanchao Xue, Hans R. Scholer,9, Qianwen Sun* and Hongjie Yao*

Abstract

R-loops modulate genome stability and regulate gene expression, but the functions and the regulatory mechanisms of R-loops in stem cell biology are still unclear. Here, we profiled R-loops during somatic cell reprogramming and found that dynamic changes in R-loops are essential for reprogramming and occurred before changes in gene expression. Disrupting the homeostasis of R-loops by depleting RNaseH1 or catalytic inactivation of RNaseH1 at D209 (RNaseH1^D209N) blocks reprogramming. Sox2, but not any other factor in the Yamanaka cocktail, overcomes the inhibitory effects of RNaseH1 activity loss on reprogramming. Sox2 interacts with the reprogramming barrier factor Ddx5 and inhibits the resolvase activity of Ddx5 on R-loops and thus facilitates reprogramming. Furthermore, reprogramming efficiency can be modulated by dCas9-mediated RNaseH1/RNaseH1^D209N targeting the specific R-loop regions. Together, these results show that R-loops play important roles in reprogramming and shed light on the regulatory module of Sox2/Ddx5 on R-loops during reprogramming.

Article link：https://advances.sciencemag.org/content/6/24/eaba0777