Science, 25 August, 2023, DOI：https://doi.org/10.1126/science.add8673

Structural insights into histone binding and nucleosome assembly by chromatin assembly factor-1

Chao-Pei Liu, Zhenyu Yu, Jun Xiong, Jie Hu, Aoqun Song, Dongbo Ding, Cong Yu, Na Yang, Mingzhu Wang, Juan Yu, Peini Hou, Kangning Zeng, Zhenyu Li, Zhuqiang Zhang, Xinzheng Zhang, Wei Li, Zhiguo Zhang, Bing Zhu, Guohong Li, and Rui-Ming Xu

Abstract

Inheritance of chromatin structure during cell division is essential for genome integrity and epigenetic inheritance. DNA replication disrupts parental nucleosomes, and nascent nucleosomes must be assembled on replicated DNA. Half of the histones for the replication-coupled nucleosome assembly are of parental origin, and the other half are newly synthesized. Chromatin assembly factor-1 (CAF-1) is an evolutionarily conserved heterotrimeric protein complex responsible for the deposition of newly synthesized histones H3 and H4 onto DNA. A mechanistic understanding of the structure and function of CAF-1 is needed to fully comprehend the principles of chromatin inheritance.

Article link：https://www.science.org/doi/10.1126/science.add8673