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Surface Ligand Engineering Ruthenium Nanozyme Superior to Horseradish Peroxidase for Enhanced Immunoassay, Adv Mater, 22 Apr 2023

Updated: 2023-04-22

Advanced Materials, 22 April, 2023, DOI:https://doi.org/10.1002/adma.202300387

 

Surface Ligand Engineering Ruthenium Nanozyme Superior to Horseradish Peroxidase for Enhanced Immunoassay


Huizhen Fan, Jiajia Zheng, Jiaying Xie, Juewen Liu, Xingfa Gao, Xiyun Yan, Kelong Fan, Lizeng Gao

 

Abstract


Nanozymes have great potential to be used as an alternative to natural enzymes in a variety of fields. However, low catalytic activity compared with natural enzymes limits their practical use. It is still challenging to design nanozymes comparable to their natural counterparts in terms of the specific activity. In this study, a surface engineering strategy is employed to improve the specific activity of Ru nanozymes using charge-transferrable ligands such as polystyrene sulfonate (PSS). PSS-modified Ru nanozyme exhibits a peroxidase-like specific activity of up to 2820 U mg-1, which is twice that of horseradish peroxidase (1305 U mg-1). Mechanism studies suggest that PSS readily accepts negative charge from Ru, thus reducing the affinity between Ru and ·OH. Importantly, the modified Ru-peroxidase nanozyme is successfully used to develop an immunoassay for human alpha-fetoprotein and achieves a 140-fold increase in detection sensitivity compared with traditional horseradish-peroxidase-based enzyme-linked immunosorbent assay. Therefore, this work provides a feasible route to design nanozymes with high specific activity that meets the practical use as an alternative to natural enzymes.

 

Article link:https://onlinelibrary.wiley.com/doi/10.1002/adma.202300387

 

 

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