Home > Faculty

Guangxia Gao, Ph.D, Prof.

Principal Investigator
Chinese Academy of Sciences Key Laboratory of Infection and Immunity
Chair of CAS Key Laboratory of Infection and Immunity


Research Interests: Mechanistic studies on virus-host interactions


Email: gaogx@ibp.ac.cn


Tel: 010-64888545


Address: 15 Datun Road, Chaoyang District, Beijing, 100101, China


Chinese personal homepage


Biography

1988             Bachelor of Science, Department of Biochemistry, Beijing University

1995             Doctor of Philosophy, Department of Biochemistry, Columbia University, USA

1995 - 1999  Postdoctoral fellow, Biochemistry Department, Howard Hughes Medical Institute, Columbia University

1999 - 2001  Associate Research Scientist, Biochemistry Department, Columbia University

2001 -           Professor, Chinese Academy of Sciences

Awards
Membership in Academies & Societies
Research Interests

Our research mainly focuses on the molecular mechanisms underlying virus-host interactions. Specifically, we identify new host factors that participate in the regulation of virus replication, and explore the underlying mechanisms. We used to work on the Zinc-finger Antiviral Protein (ZAP), which specifically binds to viral mRNA, represses its translation and recruits RNA degradation machinery to degrade the RNA. We recently identified a host antiviral factor, Shiftless, which inhibits programmed -1 ribosomal frameshifting. Shiftless inhibits the frameshiftling process of not only viral RNAs also cellular mRNAs. These results were published in high-profile journals such as Science, Cell, Cell Host & Microbe, EMBO J and PNAS. Our research in the near future will focus on two aspects: (1) identification and mechanistic analyses of novel host factors involved in HIV, SARS-CoV-2 replication (2) identification of cellular mRNAs that contain -1PRF signals and analyses of their functions in vivo using Shiftless knockout mice as a tool.

Grants
Selected Publications

1. Luo, X., Wang, X., Gao, Y., Zhu, J., Liu, S., Gao, G.*, Gao, P.*. (2020) Molecular Mechanism of RNA Recognition by Zinc-Finger Antiviral Protein. Cell Rep 30, 46-52 e44

2. Wang, X., Xuan, Y., Han, Y., Ding, X.,Ye, K., Yang, F., Gao, P., Goff, SP., Gao, G.(2019) Regulation of HIV-1 Gag-Pol Expression by Shiftless, an Inhibitor of Programmed -1 Ribosomal Frameshifting.Cell 176(3): 625-635.

3. Luo, X., Yang, W., Gao, G. (2018) SUN1 regulates HIV-1 nuclear import in a manner dependent on the interaction between the viral capsid and cellular cyclophilin A. J Virol 92(13). pii: e00229-18.

4. Zhang, X., Yang, W., Wang, X., Zhang, X., Tian, H., Deng, H., Zhan, L., Gao G.(2018) Identification of new type I interferon-stimulated genes and investigation of their involvement in IFN-β activation. Protein Cell 9(9):799-807.

5. Chen S, Gao G*. (2017) MicroRNAs recruit eIF4E2 to repress translation of target mRNAs. Protein & Cell 8(10), 750-761.

6. Zheng X., Wang X., Tu F, Wang Q., Fan Z, Gao G*. (2017) TRIM25 Is Required for the Antiviral Activity of Zinc Finger Antiviral Protein. J Virol 91(9). pii: e00088-17.

7. Tang Q, Wang X, Gao G*. (2016) ZAPS inhibits influenza A viral protein expression and is antagonized by the virus encoded NS1. J Virol. Nov 2. pii: JVI.01909-16.

8. Wang X, Li M, Zhao J, Li S, MacDonald M, Rice C, Gao X, Gao G*. (2016) Sindbis virus can exploit a host antiviral protein to evade immune surveillance. J Virol 90(22): p10247-10258.

9. Wang Q, Zhang X, Han Y, Wang X*, Gao G*. (2016) M2BP inhibits HIV-1 virion production in a vimentin filamentsdependent manner. Scientific Reports. Sep 8;6:32736.

10. Tao X, Gao G*. (2015) Tristetraprolin Recruits Eukaryotic Initiation Factor 4E2 To Repress Translation of AU-Rich Element-Containing mRNAs. Mol Cell Biol. Nov 15;35(22):3921-32.

11. Mu X, Fu Y, Zhu Y, Wang X, Xuan Y, Shang H, Goff SP, Gao G*. (2015) HIV-1 exploits RuvB-like 2 to balance viral protein expression. Cell Host & Microbe. Aug 12;18(2):233-42.

12. Mu X, Li W, Wang X, Gao G*. (2013) YB-1 stabilizes HIV-1 genomic RNA and enhances viral production. Protein & Cell. Aug;4 (8):591-7

13. Xuan Y, Gong D, Qi J, Han C, Deng H, Gao G*. (2013) ZAP inhibits murine gammaherpesvirus-68 ORF64 expression and is antagonized by RTA, J Virol. 87 (5): 2735-43.

14. Zhu Y, Wang X, Goff SP, Gao G*. (2012) Translational repression precedes and is required for ZAP-mediated mRNA decay. EMBO J. 31 (21):4236-46.

15. Li W, Wang X, Gao G*. (2012) Expression of YB-1 enhances production of murine leukemia virus vectors by stabilizing genomic viral RNA. Protein & Cell. Dec;3(12):943-9.

16. Xuan Y, Liu L, Shen S, Deng H, Gao G*. (2012) Zinc-finger antiviral protein inhibits murine gammaherpesvirus-68 M2 expression and regulates viral latency in cultured cells. J Virol. 86 (22):12431-4.

17. Wang, X., Tu, F., Zhu, Y., and Gao G*. (2012) Zinc-finger antiviral protein inhibits XMRV infection. PLoS One 7, e39159

18. Sun L, Lv F, Guo X, Gao G*. (2012) Glycogen synthase kinase 3β (GSK3β) modulates antiviral activity of zinc-finger antiviral protein (ZAP). J Biol Chem. 287 (27): 22882-8.

19. Chen S, Xu Y, Zhang K, Wang X, Sun J, Gao G*, Liu Y*. (2012) Structure of N-terminal domain of ZAP indicates how a zinc-finger protein recognizes complex RNA. Nat Struct Mol Biol. 19 (4): 430-5.

20. Zhu Y, Chen G, Lv F, Wang X, Ji X, Xu Y, Sun J, Wu L, Zheng Y, Gao G*. (2011) Zinc-finger antiviral protein inhibits HIV-1 infection by selectively targeting multiply spliced viral mRNAs for degradation. Proc Natl Acad Sci USA. Sep 20;108 (38):15834-9.

21. Ding J, Su L, Gao G*. (2011) Hrs inhibits citron kinase-mediated HIV-1 budding via its FYVE domain. Protein & Cell. Jun;2 (6):470-6.

22. Mai Y, Gao G*. (2010) Expression of IMP1 Enhances Production of Murine Leukemia Virus Vector by Facilitating Viral Genomic RNA Packaging. PLoS ONE. Dec 29; 5 (12):e15881.

23. Ye P, Liu S, Zhu Y, Chen G, Gao G*. (2010) DEXH-Box Protein DHX30 is required for optimal function of the zinc-finger antiviral protein. Protein & Cell. Vol.1 (10),956-964.

24. Huang Z, Wang X, Gao G*. (2010) Analyses of SELEX-derived ZAP-binding RNA aptamers suggest that the binding specificity is determined by both structure and sequence of the RNA. Protein & Cell. Vol.1(8), 752-759.

25. Wang X, Lv F, Gao G*. (2010) Mutagenesis analysis of the zinc-finger antiviral protein. Retrovirology. Mar 13;7:19.

26. Xu J, Liu S, Xu Y, Tian B, Gao G*. (2009) Identification of the nonstructural protein 4B of hepatitis C virus as a factor that inhibits the antiviral activity of interferon-alpha. Virus Research. 141(1): 55–62.

27. Zhu Y, Gao G*. (2008) ZAP-mediated mRNA degradation.  RNA Biology 5 (2): 65-7 (Invited minireview)

28. Chen G, Guo X, Lv F, Gao G*. (2008) p72 DEAD box RNA helicase is required for optimal function of the Zinc-finger antiviral protein. Proc Natl Acad Sci USA. 105 (11): 4352-7.

29. Guo X., Ma J., Sun J., and Gao G*. (2007) The Zinc-finger Antiviral Protein Recruits the RNA Processing Exosome to Degrade Target RNAs. Proc Natl Acad Sci USA. Vol. 104 (1): 151-6

30. Duan J, Ji X, et al, Gao G*, Yan X* (2006) A human neutralizing antibody against a conformational epitope shared by oligomeric S1 protein. Antiviral Therapy 11(1): 117-23

31. Liu S, Goff SP and Gao G* (2006) Gln84 of Moloney murine leukemia virus reverse transcriptase regulates the incorporation rates of ribonucleotides and deoxyribonucleotides. FEBS Letters 580: 1497-1501.

32. Guo X, Carroll JW, Macdonald MR, Goff SP, Gao G*. (2004) The zinc finger antiviral protein directly binds to specific viral mRNAs through the CCCH zinc finger motifs. J Virol. Dec;78(23):12781-7.

33. Liu L, Chen G, Ji X, Gao G* (2004). ZAP is a CRM1-dependent nucleocytoplasmic shuttling protein. Biochem Biophys Res Commun. Aug 27;321(3):517-23.

34. Gao G, Goff SP (2004) Isolation of suppressor genes that restore retrovirus susceptibility to a virus-resistant cell line. Retrovirology. Sep 28;1 (1):30.

35. Gao, G.,Guo,X and Goff, S.P. (2002). Inhibition of Retroviral RNA Production by ZAP, a CCCH-type Antiviral Protein. Science 297:1703-1706.

 

(From Guangxia Gao, December 28, 2021)

 

Contact Us

Tel: 010-64889872

E-Mail: webadmin@ibp.ac.cn

Address: No 15 Datun Road, Chaoyang District, Beijing

Postcode: 100101