1997 - 2001  Wuhan University, B.S.

2001 - 2006  Institute of Biophysics, Chinese Academy of Sciences,Ph.D

2006  -2018  Institute of Biophysics, Chinese Academy of Sciences, Assistant Prof./Associate Prof.

We are focusedon the proteins involved in pathogen infection and drug resistance. We are studying on the Type 7 Secretion System (T7SS) in Mycobacterium tuberculosis and the effector proteins secreted by T7SS. We are also studying on the molecular mechanisms of the drug resistance in M. tuberculosis and other pathogens and the structureof the potential drug targets. As well, we are interesting in the regulation mechanism of the bacteria movement.

1. Yang K^#, Li DF^#, Wang X, Liang J, Sitia R, Wang CC*, Wang X*. Crystal Structure of the ERp44-Peroxiredoxin 4 Complex Reveals the Molecular Mechanisms of Thiol-Mediated Protein Retention. Structure. 2016 Oct 4;24(10):1755-1765.

2. Qi C#,  Li DF^#, Feng L, Hou Y, Sun H, Wang DC*, Liu W*.Biochemical and structural characterization of a novel ubiquitin-conjugating enzyme E2 from Agrocybeaegeria reveals Ube2w family-specific properties. Sci Rep. 2015 Nov 3;5:16056.

3. Zhang XL#, Li DF^#, Fleming J, Wang LW, Zhou Y, Wang DC, Zhang XE*, Bi LJ*.Core component EccB1 of the Mycobacterium tuberculosis type VII secretion system is a periplasmic ATPase. FASEB J. 2015 Dec;29(12):4804-14.

4. Li WJ#, Li DF^#, Hu YL, Zhang XE, Bi LJ*, Wang DC*.Crystal structure of L,D-transpeptidase LdtMt2 in complex with meropenem reveals the mechanism of carbapenem against Mycobacterium tuberculosis. Cell Res. 2013 May;23(5):728-31.

5. Li DF^#, Zhang JY#, Hou YJ, Liu L, Hu Y, Liu SJ*, Wang DC*, Liu W*.Structures of aminophenol dioxygenase in complex with intermediate, product and inhibitor. Acta Crystallogr D Biol Crystallogr. 2013 Jan;69(Pt 1):32-43.

6. Zhang X^#, Zhang J^#, Zhang R, Guo Y, Wu C, Mao X, Guo G, Zhang Y, Li D*, Zou Q*. Structural, enzymatic and biochemical studies on Helicobacter pylori arginase. Int J Biochem Cell Biol. 2013 May;45(5):995-1002.

7. Zhang J^#, Zhang X^#, Zhang R, Wu C, Guo Y, Mao X, Guo G, Zhang Y, Wang DC, Li D*, Zou Q*. Modeling studies with Helicobacter pylori octaprenyl pyrophosphate synthase reveal the enzymatic mechanism of trans-prenyltransferases. Int J Biochem Cell Biol. 2012 Dec;44(12):2116-23.

8. Bian CF, Zhang Y, Sun H, Li DF*, Wang DC*. Structural basis for distinct binding properties of the human galectins to Thomsen-Friedenreich antigen. PLoS One. 2011;6(9):e25007. Epub 2011 Sep 20.

9. Li DF^#, Zhang N^#, Hou YJ, Huang Y, Hu Y, Zhang Y, Liu SJ*, Wang DC*. Crystal structures of the transcriptional repressor RolR reveals a novel recognition mechanism between inducer and regulator. PLoS One. 2011 May 3;6(5):e19529.

10. Zhang J^#, Zhang X^#, Wu C, Lu D, Guo G, Mao X, Zhang Y, Wang DC, Li D*, Zou Q*.Expression, purification and characterization of arginase from Helicobacter pylori in its apo form. PLoS One. 2011;6(10):e26205.

11. FengL, Sun H, Zhang Y, Li DF* & Wang DC*. Structural insights into the recognition mechanism between an antitumor galectin AAL and the Thomsen-Friedenreich antigen. FASEB J. 2010 Oct;24(10):3861-8.

12. Yang N^#, Li DF^#, Feng L, Xiang Y, Liu W*, Sun H*, Wang DC*. Structural basis for the tumor cell apoptosis-inducing activity of an antitumor lectin from the edible mushroom Agrocybeaegerita. J Mol Biol. 2009 Apr 3;387(3):694-705

13. Li DF, Jiang P, Zhu DY, Hu Y, Max M and Wang DC. Crystal structure of Mabinlin II: a novel structural type of sweet proteins and the main structural basis for its sweetness, Journal of Structural Biology, 2008, 162(1):50-62.

14. Li YF^#, Li DF^#, Zeng ZH* & Wang DC*. Trimeric structure of the wild soluble chloride intracellular ion channel CLIC4 Observed in Crystals. BiochemBiophys Res Commun. 2006, 343, 1272-1278.

(From De-Feng Li, February 16, 2017)