2003.09 - 2007.06  B.S. on Bioengineering, Beihang University

2007.09 - 2012.06  Ph.D. on Biophysics, Institute of Biophysics, CAS

2012.07 - 2013.12  Assistant Professor, Institute of Biophysics, CAS

2014.01 - 2019.12  Associate Professor, Institute of Biophysics, CAS

2016.11 - 2017.01  Visiting Scholar, Janelia Research Campus, HHMI

2020.01 - 2023.03  Professor, Institute of Biophysics, CAS

Tools and technologies development in bio-imaging (super-resolution imaging, in-vivo deep imaging, long-term multi-dimensional imaging, multimodal imaging) to solve biological questions regarding the assembly mechanism of protein machinery, the structure and function of the neural circuits, detection and evaluation of tumors etc.

The recent progresses and on-going work are as follow:

1. Development of photo-controllable fluorescent proteins for a variety of super-resolution fluorescence microscopy including: a series of photo-switchable fluorescent proteins (mGeos) with multiple application values; rational designed photo-convertible fluorescent protein (mEos3) for application in photoactivated localization microscopy (PALM); photo-switchable fluorescent protein (Skylan-NS) that are suitable for nonlinear structure illumination microscopy (PA NL -SIM) of living cell.

2. Development of the next generation photo-controllable fluorescent protein (pcStar) and single molecule-guided Bayesian localization microscopes (SIMBA and Quick-SIMBA) for live cell super-resolution microscopy.

3. Development of fluorescent proteins in super-resolution light-electron microscopy (mEosEM) for the structure and mechanism study of protein machinery.

4. Development of near-infrared fluorescent proteins and imaging algorithms for two-photon/photoacoustic imaging to detect and evaluate tumors in small living animals.

5. Development of calcium-based probes for two-photon light-sheet microscopy to construct the whole-brain neural circuit in small model organisms such as zebrafish

1. Fu Z.^#, Peng D.^#, Zhang M.^#, *, Xue F., Zhang R., He W., Xu T.*, Xu P.*. mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM. Nature Methods 2020, 17(1): 55-58.

2. Zhang M.^#, Fu Z.^#, Li C.^#, Liu A., Peng D., Xue F., He W., Gao S., Xu F., Xu D., Yuan L., Zhang F., Xu Z., Xu T., Xu P.. Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein. Nano Letters 2020, 20(4): 2197-2208.

3. Xu F.^#, Zhang M.^#, He W., Han R., Xue F., Liu, Z., Zhang F., Lippincott-Schwartz J., Xu P.. Live cell single molecule-guided Bayesian localization super resolution microscopy. Cell Research 2017; 27: 713-716.

4. Zhang X.^#, Zhang M.^#, Li D^#, He W., Peng J., Betzig E., Xu P.. Highly photostable, reversibly photoswitchable fluorescent protein with high contrast ratio for live-cell superresolution microscopy. Proc Natl Acad Sci U S A 2016; 113: 10364-10369.

5. Zhang M, Fu Z, Xu P. Extending the spatiotemporal resolution of super-resolution microscopies using photomodulatable fluorescent proteins. Journal of Innovative Optical Health Sciences. 2016, 9(3).

6. Han L., Zhao Y., Zhang X., Peng J., Xu P.*, Huan S. *, Zhang M.*. RFP tags for labeling secretory pathway proteins. Biochemical and Biophysical Research Communications 2014; 447: 508-512. 

7. Li D, Shao L, Chen B-C, Zhang X, Zhang M, Moses B, Milkie D E, Beach J R, Hammer J A, Pasham M. Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics. Science. 2015, 349, aab3500.

8. Zhang M^#, Chang H^#, Zhang Y^#, Yu J, Wu L, Ji W, Chen J, Liu B, Lu J, Liu Y, Zhang J, Xu P, and Xu T. Rational design of true monomeric and bright photoconvertible fluorescent proteins. Nature Methods. 2012, May 13; 9(7):727-9.

9. Chang H^#, Zhang M^#, Ji W^#, Chen J, Zhang Y, Liu B, Lu J, Zhang J, Xu P, and Xu T. A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications. Proc Natl Acad Sci U S A. 2012, 109(12):4455-4460.

10. Liu L^#, Zhang M^#, Xia Z, Xu P, Chen L, Xu T. Caenorhabditiselegans ciliary protein NPHP-8, the homologue of human RPGRIP1L, is required for ciliogenesis and chemosensation. Biochemical and Biophysical Research Communications. 2011, 410 (3):626-631.

(From Mingshu Zhang, December 28, 2020)