On Sept 11, Professor Jan Ellenberg from the European Molecular Biology Laboratory (EMBL), invited by Professor Li Dong, visited IBP and gave a BEI Shizhang lecture entitled "Imaging technologies that enable Quantitative analysis of cellular processes across scales".

Professor Ellenberg first introduced the important applications of different microscopic imaging techniques in organelles, cells and individuals across the scale. Using advanced optical imaging techniques and image data analysis methods can help biologists solve important organisms.

He introduced the process of remodeling the nuclear pore complex in the late stage of cell division by FIB-SEM. In the late stage of mitosis and interphase, the nuclear pore complex has different dynamic assembly mechanisms. He also observed the nuclear pore complex and found that the nuclear pore complex skeletal protein Nups conformation is flexible by using Single-molecule localized super-resolution microscopy STORM.

Afterwards, Professor Ellenberg introduced the protein network map during cell division and obtained a dynamic localization network of cell division related proteins, by using 4D imaging of mitotic cells and data reconstruction analysis.

Finally, Prof. Jan Ellenberg introduced Lattice Light Sheet Microscopy (LLSM), which can reduce photo toxicity damage to embryos. His team studied embryonic cell division and found that the first cleavage in mammalian fertilized eggs was performed by a dual-spindle and the maternal and paternal genomes were pulled by two spindles via using this technique. The discovery has changed the perception of mitosis and highlights the importance of advanced optical microscopy imaging for life science research.

Professor Jan Ellenberg is senior researcher and head of the Cell Biology & Biophysics Unit. For over 20 years, he has been interested in cell division and nuclear organization, including systematic analysis of mitosis, structure and assembly of nuclear pore complexes, chromatin organization, and chromosome formation and separation in mitosis.

Professor Jan Ellenberg is giving the lecture.

Professor Jan Ellenberg and Professor Li Dong