PNAS, 3 March, 2021, DOI：https://doi.org/10.1073/pnas.2022614118

Structural insights into membrane remodeling by SNX1

Yan Zhang, Xiaoyun Pang, Jian Li, Jiashu Xu, Victor W. Hsu and Fei Sun

Abstract

The sorting nexin (SNX) family of proteins deform the membrane to generate transport carriers in endosomal pathways. Here, we elucidate how a prototypic member, SNX1, acts in this process. Performing cryoelectron microscopy, we find that SNX1 assembles into a protein lattice that consists of helical rows of SNX1 dimers wrapped around tubular membranes in a crosslinked fashion. We also visualize the details of this structure, which provides a molecular understanding of how various parts of SNX1 contribute to its ability to deform the membrane. Moreover, we have compared the SNX1 structure with a previously elucidated structure of an endosomal coat complex formed by retromer coupled to a SNX, which reveals how the molecular organization of the SNX in this coat complex is affected by retromer. The comparison also suggests insight into intermediary stages of assembly that results in the formation of the retromer-SNX coat complex on the membrane.

Article link：https://www.pnas.org/content/118/10/e2022614118